Granzyme B (and A) are serine proteases that can mediate apoptotic signaling in CTL (cytotoxic T lymphocytes) and NK (natural killer) cells. Both versions are synthesized to be inactive proenzymes that can be stored within the cytolytic granules and released during degradation by effector cells. This antibody can be very useful to localize the lytic granules that contain Granzyme B and to help characterize CTL and NK cells.
It is designed to be used for research and has no clone. Likewise, the immunogen is the synthetic peptide from the human Granzyme B N-terminus. The isotype is the Rabbit IgG, and it has no determine epitope. Likewise, it has a molecular weight of 32kDa.
Applications
Granzyme B is designed to be used with Immunohistochemistry applications. To prepare your specimens, you should use formalin-fixed or paraffin-embedded tissues. Likewise, deparaffinized slides are required. You can clean them using xylene or its alternative, as well as graded alcohols.
When you choose the concentrated formula for the product, you will need to dilute the antibody using a ratio of 1:100. Likewise, you can find pre-diluted versions available to make it easier, depending on your protocols and methods.
To retrieve the antigen, you’ll need to boil tissue sections in a 10mM citrate buffer with a pH of 6.0. Do this for 10 minutes and allow it to cool to room temperature for at least 20 minutes. Immediately afterward, you should incubate the concoction for 10 minutes at room temperature. It is recommended to rinse slides with PBS-Tween between steps.
The positive control for this antibody is the tonsil with cellular localization happening in the Granular cytoplasm.
Granzyme B can help you determine which cells are CTL and NK, as well as distinguish them from others. Visit Spring Bioscience now to learn more.
