How To Use Granzyme B For Testing

by | Oct 27, 2017 | Science and Technology

Recent Articles



Granzyme A and B are serine proteases that can help with apoptotic signaling in the natural killer cells and cytotoxic T lymphocytes. Both options are created as inactive proenzymes and are stored in the cytolytic granules and released during degradation by effector cells. This antibody can be useful for localization of the lytic granules that contain Granzyme B and to help characterize the activated NK and CTL cells.

More Information

Granzyme B does not have a clone, and the immunogen is the synthetic peptide from the human antibody with the same name, found in the N-terminus. It has an undetermined epitope, and the isotype is the Rabbit IgG. Its molecular weight is 32kDa.


Granzyme B can be used with Immunohistochemistry applications. You can use paraffin-embedded or Formalin-fixed tissues for this antibody. Likewise, deparaffinized slides should be used with graded alcohols, xylene, and its alternatives.

You’ll find a pre-diluted formula that will work for IHC applications, as long as you need the traditional ratio. If you must use a different protocol or method, you may want to consider the concentrated format and dilute it appropriately, usually in a ratio of 1:100.

To retrieve the antigen, you should boil the tissue section in a 10mM citrate buffer with a pH of 6.0. This should be done for ten minutes and then cooled to room temperature for 20 minutes. The antibody should also be incubated for ten minutes at room temperature. You can wash the slides using a PBS/0.05% Tween solution, which is recommended.

The positive control is the tonsil with cellular localization happening in the granular cytoplasm.

Granzyme B is an antibody that can help with a variety of research needs. Visit Spring Bioscience today to learn more and purchase some for your laboratory.